Overexpression constructs

The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)

HyEED (LigD)

Transgenic construct:

  1. Vector:
    • HyEED (LigD)
  2. Backbone:
    • hoT_G
  3. Design:
    • actinP∷HyEED–eGFP
  4. Overexpressed gene:
    • HyEED
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • EED-OE D3: ISC, Nb GFP

Reference:

Khalturin et al., Dev Biol 2007

mHym301A

Transgenic construct:

  1. Vector:
    • mHym301A
  2. Backbone:
    • hoT_G
  3. Design:
    • actinP∷mHym301A–eGFP
  4. Overexpressed gene:
    • mHym301A
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • A14: ecto GFP

Reference:

Khalturin et al., PLoS Biol 2008

Peri1a

Transgenic construct:

  1. Vector:
    • Peri1a
  2. Backbone:
    • hoT_G
  3. Design:
    • actinP::Peri1a–eGFP–Peri1a
  4. Overexpressed gene:
    • Peri1a
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • Peri-OE A11: ecto GFP

Reference:

Fraune et al., PNAS 2010

bCat-OE

Transgenic construct:

  1. Vector:
    • bCat-OE
  2. Backbone:
    • pHyvec4
  3. Design:
    • actinP∷bCat(delta1-138)–eGFP
  4. Overexpressed gene:
    • HyβCat
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • bCat-OE: ecto GFP

Reference:

Gee et al., Dev.Biol 2010

FoxO-OE

Transgenic construct:

  1. Vector:
    • FoxO-OE
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷FoxO–eGFP
  4. Overexpressed gene:
    • FoxO
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • A4: ISC, Nb GFP
    • C2: ecto, ISC, Nb GFP
    • C6: ecto GFP

Reference:

Boehm et al., PNAS 2012

HyLMN-OE

Transgenic construct:

  1. Vector:
    • HyLMN-OE
  2. Backbone:
    • LigAF
  3. Design:
    • actP::eGFP–HyLMN
  4. Overexpressed gene:
    • HyLMN
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • N5: ecto GFP
    • N5 empty: no GFP
    • N13: ecto GFP
    • N13 empty: no GFP

Reference:

Klimovich et al., Aging 2018

HyLMNdCaaX-OE

Transgenic construct:

  1. Vector:
    • HyLMNdCaaX-OE
  2. Backbone:
    • LigAF
  3. Design:
    • actP::eGFP–HyLMNdCaaX
  4. Overexpressed gene:
    • hyLMNdCaaX box
  5. Sequence:
    • FASTA-file
  6. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • B3: endo GFP
    • B3 emty: no GFP
    • C11: endo GFP
    • C11 empty: no GFP

Reference:

Klimovich et al., Aging 2018