The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)
hoT_G
Transgenic construct:
- Vector:
- hoT_G
- Backbone:
- pUC19
- Design:
- actinP::eGFP
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- endo-2: endo GFP
- ICY: ISC, Nv, Nb, Nc, Gd, GL GFP
- ecto-1 (A8): ecto GFP
Reference:
Wittlieb et al., PNAS 2006
Khalturin et al., Dev Biol 2007
Anton-Erxleben et al., Zoology 2009
HyDKK1/2/4 C
Transgenic construct:
- Vector:
- HyDKK1/2/4 C
- Backbone:
- hoT_G
- Design:
- HyDKK1/2/4cP::eGFP
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- DLP reporter: Gd GFP
Reference:
Siebert et al., DevBiol 2007
nb001 (LigAB)
Transgenic construct:
- Vector:
- nb001 (LigAB)
- Backbone:
- hoT_G
- Design:
- nb001P::eGFP
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- D2: Nc GFP
Reference:
Milde et al., 2009
cnnos1
Transgenic construct:
- Vector:
- cnnos1
- Backbone:
- NA
- Design:
- cnnos1P::eGFP//actinP::DsRED2
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- N4: ISC, Nb GFP, ecto DsRED
Reference:
Boehm and Bosch, Zoology 2012