Reporter constructs

The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)

hoT_G

Transgenic construct:

  1. Vector:
    • hoT_G
  2. Backbone:
    • pUC19
  3. Design:
    • actinP::eGFP
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • endo-2: endo GFP
    • ICY: ISC, Nv, Nb, Nc, Gd, GL GFP
    • ecto-1 (A8): ecto GFP

Reference:

Wittlieb et al., PNAS 2006
Khalturin et al., Dev Biol 2007
Anton-Erxleben et al., Zoology 2009

HyDKK1/2/4 C

Transgenic construct:

  1. Vector:
    • HyDKK1/2/4 C
  2. Backbone:
    • hoT_G
  3. Design:
    • HyDKK1/2/4cP::eGFP
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • DLP reporter: Gd GFP

Reference:

Siebert et al., DevBiol 2007

nb001 (LigAB)

Transgenic construct:

  1. Vector:
    • nb001 (LigAB)
  2. Backbone:
    • hoT_G
  3. Design:
    • nb001P::eGFP
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • D2: Nc GFP

Reference:

Milde et al., 2009

cnnos1

Transgenic construct:

  1. Vector:
    • cnnos1
  2. Backbone:
    • NA
  3. Design:
    • cnnos1P::eGFP//actinP::DsRED2
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • N4: ISC, Nb GFP, ecto DsRED

Reference:

Boehm and Bosch, Zoology 2012