Inducible constructs

The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)

indGFP-OE

Transgenic construct:

  1. Vector:
    • indGFP-OE
  2. Backbone:
    • indGFP
  3. Design:
    • tetO-minicmvP::eGFP//actP::DsRED2
  4.  Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • AK16 indGFP: ecto GFP (+Dox), ecto DsRED
    • AK16 empty: no GFP, no DsRED

Reference:

Klimovich et al., Aging 2018

indHyLMN-KD hp405-929

Transgenic construct:

  1. Vector:
    • indHyLMN-KD hp405-929
  2. Backbone:
    • indGFP
  3. Design:
    • tetO-minicmvP::eGFP–shHyLMN405-929//actP::DsRED2
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • D1: ecto GFP (+Dox), ecto DsRED
    • D1 empty: no GFP, no DsRED
    • D10: ecto GFP (+Dox), ecto DsRED
    • D10 empty: no GFP, no DsRED

Reference:

Klimovich et al., Aging 2018

indHyLMN-KD hp1209-1707

Transgenic construct:

  1. Vector:
    • indHyLMN-KD hp1209-1707
  2. Backbone:
    • indGFP
  3. Design:
    • tetO-minicmvP::eGFP–shHyLMN1209-1707//actP::DsRED2
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • F5: ecto GFP (+Dox), ecto DsRED
    • F5 empty: no GFP, no DsRED
    • F10: ecto GFP (+Dox), ecto DsRED
    • F10 empty: no GFP, no DsRED

Reference:

Klimovich et al., Aging 2018