The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)
FoxO-KD
Transgenic construct:
- Vector:
- FoxO-KD
- Backbone:
- LigAF
- Design:
- actinP∷eGFP–shFoxO
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- A6: endo GFP
- D11a: endo GFP
- E1: endo GFP
- D11a: ecto GFP
- D11a: ecto endo GFP
- empty: no GFP
Reference:
Boehm et al., PNAS 2012
Mortzfeld et al., Front Microb 2018
MyD88-KD
Transgenic construct:
- Vector:
- MyD88-KD
- Backbone:
- LigAF
- Design:
- actinP∷eGFP–shMyD88
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- D3: ecto endo GFP
- D3 empty: no GFP
Reference:
Franzenburg et al., PNAS 2012
Arminin-KD
Transgenic construct:
- Vector:
- Arminin-KD
- Backbone:
- LigAF
- Design:
- actinP∷eGFP–shArminin
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- K3: endo GFP
- K3 empty: no GFP
Reference:
Franzenburg et al., PNAS 2013
HyPON-KD
Transgenic construct:
- Vector:
- HyPON-KD
- Backbone:
- LigAF
- Design:
- actinP∷eGFP–shHyPON
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- C1: ecto, endo GFP
- C1 empty: no GFP
Reference:
Pietschke et al, PNAS 2017
NDA-1-KD
Transgenic construct:
- Vector:
- NDA-1-KD
- Backbone:
- LigAF
- Design:
- actinP∷eGFP–shNDA-1
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- D1: Nv GFP
- D1 empty: no GFP
- E11: Nv GFP
- E11 empty: no GFP
Reference:
Augustin et al., 2017
HyLMN-hp405-929
Transgenic construct:
- Vector:
- HyLMN-hp405-929
- Backbone:
- LigAF
- Design:
- actP::eGFP–shHyLMN405-929
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- lethal
Reference:
Klimovich et al., Aging 2018
HyLMN-hp1209-1707
Transgenic construct:
- Vector:
- HyLMN-hp1209-1707
- Backbone:
- LigAF
- Design:
- actP::eGFP–shHyLMN1209-1707
- Sequence:
- FASTA-file
- Plasmid map:
Transgenic Hydra:
- Line phenotype:
- lethal
Reference:
Klimovich et al., Aging 2018