Knock-down constructs

The following constructs and transgenic lines have been developed at the transgenic hydra facility. The plasmids and transgenic animals are avaiable upon request. (see contacts)

FoxO-KD

Transgenic construct:

  1. Vector:
    • FoxO-KD
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷eGFP–shFoxO
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • A6: endo GFP
    • D11a: endo GFP
    • E1: endo GFP
    • D11a: ecto GFP
    • D11a: ecto endo GFP
    • empty: no GFP

Reference:

Boehm et al., PNAS 2012
Mortzfeld et al., Front  Microb 2018

MyD88-KD

Transgenic construct:

  1. Vector:
    • MyD88-KD
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷eGFP–shMyD88
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • D3: ecto endo GFP
    • D3 empty: no GFP

Reference:

Franzenburg et al., PNAS 2012

Arminin-KD

Transgenic construct:

  1. Vector:
    • Arminin-KD
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷eGFP–shArminin
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • K3: endo GFP
    • K3 empty: no GFP

Reference:

Franzenburg et al., PNAS 2013

HyPON-KD

Transgenic construct:

  1. Vector:
    • HyPON-KD
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷eGFP–shHyPON
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • C1: ecto, endo GFP
    • C1 empty: no GFP

Reference:

Pietschke et al, PNAS 2017

NDA-1-KD

Transgenic construct:

  1. Vector:
    • NDA-1-KD
  2. Backbone:
    • LigAF
  3. Design:
    • actinP∷eGFP–shNDA-1
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • D1: Nv GFP
    • D1 empty: no GFP
    • E11: Nv GFP
    • E11 empty: no GFP

Reference: 

Augustin et al., 2017

HyLMN-hp405-929

Transgenic construct:

  1. Vector:
    • HyLMN-hp405-929
  2. Backbone:
    • LigAF
  3. Design:
    • actP::eGFP–shHyLMN405-929
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • lethal

Reference:

Klimovich et al., Aging 2018

HyLMN-hp1209-1707

Transgenic construct:

  1. Vector:
    • HyLMN-hp1209-1707
  2. Backbone:
    • LigAF
  3. Design:
    • actP::eGFP–shHyLMN1209-1707
  4. Sequence:
    • FASTA-file
  5. Plasmid map:

Transgenic Hydra:

  1. Line phenotype:
    • lethal

Reference:

Klimovich et al., Aging 2018